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aav1 cmv egfp virus  (Vector Biolabs)


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    Structured Review

    Vector Biolabs aav1 cmv egfp virus
    Aav1 Cmv Egfp Virus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav1+cmv+egfp+virus/AAV1-GFP/pm41735509-77-45-47
    Average 95 stars, based on 12 article reviews
    aav1 cmv egfp virus - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Regulation of dendritic spines in the amygdala following sleep deprivation.
    Article Snippet: .. Viral vector injection and sleep deprivation Adult (3–4 months old) male C57BL/6J mice (6 controls, 6 sleep-deprived mice) received bilateral stereotaxic microinjections of 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. ..

    Injection:

    Article Title: Regulation of dendritic spines in the amygdala following sleep deprivation.
    Article Snippet: .. Viral vector injection and sleep deprivation Adult (3–4 months old) male C57BL/6J mice (6 controls, 6 sleep-deprived mice) received bilateral stereotaxic microinjections of 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. ..

    Virus:

    Article Title: Regulation of dendritic spines in the amygdala following sleep deprivation.
    Article Snippet: .. Viral vector injection and sleep deprivation Adult (3–4 months old) male C57BL/6J mice (6 controls, 6 sleep-deprived mice) received bilateral stereotaxic microinjections of 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. ..

    Article Title: Regulation of dendritic spines in the amygdala following sleep deprivation
    Article Snippet: .. Adult (3–4 months old) male C57BL/6J mice (6 controls, 6 sleep-deprived mice) received bilateral stereotaxic microinjections of 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. ..

    Article Title: Sleep Deprivation Alters Hippocampal Dendritic Spines in a Contextual Fear Memory Engram
    Article Snippet: .. ArcCreER T2 mice were anesthetized with 1.5% isoflurane and received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study .

    Article Title: Sleep deprivation alters hippocampal dendritic spines in a contextual fear memory engram.
    Article Snippet: .. Animals received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector per hemisphere for a total of 4 μl/animal under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl per hemisphere for a total of 4 μl/animal of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002). .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study 29.

    Bioprocessing:

    Article Title: Sleep Deprivation Alters Hippocampal Dendritic Spines in a Contextual Fear Memory Engram
    Article Snippet: .. ArcCreER T2 mice were anesthetized with 1.5% isoflurane and received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study .

    Article Title: Sleep deprivation alters hippocampal dendritic spines in a contextual fear memory engram.
    Article Snippet: .. Animals received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector per hemisphere for a total of 4 μl/animal under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl per hemisphere for a total of 4 μl/animal of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002). .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study 29.

    Control:

    Article Title: Sleep Deprivation Alters Hippocampal Dendritic Spines in a Contextual Fear Memory Engram
    Article Snippet: .. ArcCreER T2 mice were anesthetized with 1.5% isoflurane and received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002) in each hemisphere. .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study .

    Article Title: Sleep deprivation alters hippocampal dendritic spines in a contextual fear memory engram.
    Article Snippet: .. Animals received bilateral stereotaxic microinjections of a cocktail of 2 μl of AAV mCherry ChR2 viral vector per hemisphere for a total of 4 μl/animal under the control of the CMV promoter (AAV2-CMV-DIO-mCherry), and 2 μl per hemisphere for a total of 4 μl/animal of AAV1-CMV-eGFP virus (Vector Biolabs, cat# 7002). .. The virus cocktail was infused using a Nanofil 35-gauge stainless steel bevel needle (catalog # NF35BV, World Precision Instruments, Inc., Sarasota, FL) attached to a 10μl Nanofil syringe (Hamilton Company, Reno, NV) as described in our previous study 29.



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    Anterograde transsynaptic tracing from MCN and LCN to IO via SC. A , Schematic representation of anterograde transsynaptic tracing strategy. B , Transsynaptic <t>AAV1</t> expressing Cre and eGFP (cyan) was injected in the MCN ( n = 2) or LCN ( n = 2), followed by an injection of Cre-dependent mCherry (magenta) in the lateral part of SC (all animals). Bi – iv , Representative example photos of injection spots in MCN ( Bi ) and LCN ( Bii ) and subsequent transsynaptic labeling of cells in SC following injection in MCN ( Biii ) and LCN ( Biv ). Note that a small leakage to the interposed nucleus (INT) cannot be excluded in Bi and Bii , but the MCN and LCN injections never contaminated each other. C , Anterograde labeling in the IO following transsynaptic tracing; the labeling is predominantly ipsilateral to the MCN ( Ci ) and LCN ( Cii ) that were injected. Note that some transsynaptically labeled cells can be observed in the IO contralateral to MCN injection spot ( Ci ). D , Images of a fiber in the caudal MAO with varicosities (yellow arrows) positive for mCherry ( Di ), GAD67 ( Dii ), or both ( Diii ). E , Number of GAD67+ and GAD67− varicosities in cdMAO for MCN and LCN injections. Error bars represent standard deviation (SD), and dots represent number of varicosities in individual mice. F , Summary panel demonstrating the laterality of the observed inhibitory disynaptic projection from the MCN to the cdMAO and the complementary direct excitatory projection from the MCN.
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    KEY RESOURCES TABLE
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    Image Search Results


    Anterograde transsynaptic tracing from MCN and LCN to IO via SC. A , Schematic representation of anterograde transsynaptic tracing strategy. B , Transsynaptic AAV1 expressing Cre and eGFP (cyan) was injected in the MCN ( n = 2) or LCN ( n = 2), followed by an injection of Cre-dependent mCherry (magenta) in the lateral part of SC (all animals). Bi – iv , Representative example photos of injection spots in MCN ( Bi ) and LCN ( Bii ) and subsequent transsynaptic labeling of cells in SC following injection in MCN ( Biii ) and LCN ( Biv ). Note that a small leakage to the interposed nucleus (INT) cannot be excluded in Bi and Bii , but the MCN and LCN injections never contaminated each other. C , Anterograde labeling in the IO following transsynaptic tracing; the labeling is predominantly ipsilateral to the MCN ( Ci ) and LCN ( Cii ) that were injected. Note that some transsynaptically labeled cells can be observed in the IO contralateral to MCN injection spot ( Ci ). D , Images of a fiber in the caudal MAO with varicosities (yellow arrows) positive for mCherry ( Di ), GAD67 ( Dii ), or both ( Diii ). E , Number of GAD67+ and GAD67− varicosities in cdMAO for MCN and LCN injections. Error bars represent standard deviation (SD), and dots represent number of varicosities in individual mice. F , Summary panel demonstrating the laterality of the observed inhibitory disynaptic projection from the MCN to the cdMAO and the complementary direct excitatory projection from the MCN.

    Journal: eNeuro

    Article Title: Disynaptic Inhibitory Cerebellar Control Over Caudal Medial Accessory Olive

    doi: 10.1523/ENEURO.0262-23.2023

    Figure Lengend Snippet: Anterograde transsynaptic tracing from MCN and LCN to IO via SC. A , Schematic representation of anterograde transsynaptic tracing strategy. B , Transsynaptic AAV1 expressing Cre and eGFP (cyan) was injected in the MCN ( n = 2) or LCN ( n = 2), followed by an injection of Cre-dependent mCherry (magenta) in the lateral part of SC (all animals). Bi – iv , Representative example photos of injection spots in MCN ( Bi ) and LCN ( Bii ) and subsequent transsynaptic labeling of cells in SC following injection in MCN ( Biii ) and LCN ( Biv ). Note that a small leakage to the interposed nucleus (INT) cannot be excluded in Bi and Bii , but the MCN and LCN injections never contaminated each other. C , Anterograde labeling in the IO following transsynaptic tracing; the labeling is predominantly ipsilateral to the MCN ( Ci ) and LCN ( Cii ) that were injected. Note that some transsynaptically labeled cells can be observed in the IO contralateral to MCN injection spot ( Ci ). D , Images of a fiber in the caudal MAO with varicosities (yellow arrows) positive for mCherry ( Di ), GAD67 ( Dii ), or both ( Diii ). E , Number of GAD67+ and GAD67− varicosities in cdMAO for MCN and LCN injections. Error bars represent standard deviation (SD), and dots represent number of varicosities in individual mice. F , Summary panel demonstrating the laterality of the observed inhibitory disynaptic projection from the MCN to the cdMAO and the complementary direct excitatory projection from the MCN.

    Article Snippet: For the transsynaptic experiments in wild types, we used adeno-associated virus AAV1-CMV-HI-eGFP-Cre-WPRESV40 (titer 8 × 10 12 vg/ml, 105545-AAV1; Addgene) that expresses Cre and GFP in the nucleus, while applying AAV9-hsyn-DIO-mCherry (>1 × 10 13 vg/ml, No 50459-AAV9; Addgene) in the SC as a Cre-dependent tracer.

    Techniques: Expressing, Injection, Labeling, Standard Deviation

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Lateral septum as a melanocortin downstream site in obesity development

    doi: 10.1016/j.celrep.2023.112502

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: In wild type mice, the AAV1-CMV-Cre-eGFP virus (Addgene, Plasmid #105545) was injected into the PVH (bilateral,150 nL/each) to achieve anterograde tracing, and followed by bilateral LSv injection with 200 nL AAV-Flex-CtGluR1-P2A-mCherry or control AAV-hSyn-DIO-mCherry vectors (Addgene, Plasmid #50459).

    Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Software